Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae
雜志:Fermentation-basel
出版年月:2023-7
摘要(英譯中):了解米曲霉的光響應機制對釀造和余料處理行業制定有效的光調控方案至關重要。雖然已知連續光照會限制米曲霉的生長,但米曲霉對含光照期的光照的敏感性知之甚少。在這項研究中,我們使用脈沖波調制(PWM)技術產生了9種脈沖藍光(PBL)處理,這些脈沖藍光具有不同的峰值光強度和頻率。然后我們比較了連續藍光(CBL)對米曲霉的影響。研究結果表明,GDMCC 3.31米曲霉在光劑量和平均光強恒定不變的情況下,其菌絲在特定峰值強度和頻率的PBL下生長速度快,且分生孢子產量多。其中,兩種PBL處理(PBL-20_40%_1 Hz和PBL-400_20%_10 kHz)下的菌落直徑和分生孢子數分別是CBL下米曲霉的1.13倍和1.22倍。這種不同的反應可能主要歸因于米曲霉對自然界光暗循環的適應。此外,還發現峰值光強和頻率之間存在交互作用。本研究將脈沖波調制作為影響米曲霉光響應的新因素,并將其應用于發酵光調節方法的開發中。
摘要圖:
Figure 3. Colony diameters upon PBL and CBL treatments. Subfigures (a), (b), and (c) are the results of the mycelium growth under PBL with different peak intensities if the frequency was held constant. Subfigures (d), (e), and (f) are the results of the mycelium growth under PBL with different frequencies if the peak intensity was kept unchanged. The symbols *, **, ***, and **** in the figure represent P<0.05, P<0.01, P<0.001, and P<0.0001, respectively. The label PL-100_80%_1 Hz represents a PBL with a peak intensity of 100 μmol×m-2s-1, a duty ratio of 80%, and a frequency of 1 Hz. Other labels are shown in a similar way.